-Teacher Profile-

Name: Tung-Yi Lin

Title: Associate Professor

Tel: 02-28267178 (office) / 02-2826700#66425(lab)

Email: biotungyi@gmail.com / tylin99@nycu.edu.tw

Current position:

Associate Professor,

Institute of Traditional Medicine, NYCU               

Biomedical Industry Ph.D. Program, NYCU

Program in Molecular Medicine, NYCU

 Experience:

2020-2021 Assistant Professor, Biomedical Industry Ph.D. Program, NYCU

2019-2021 Assistant Professor, Program in Molecular Medicine, NYCU

2018-2021 Assistant Professor, Institute of Traditional Medicine, NYMU

2017-2018 Postdoctoral Researcher, National Research Institute of Chinese Medicine

Education:

Program in Molecular Medicine (Ph.D.)

National Yang Ming University and Academia Sinica, Taipei, Taiwan.

Department of Biotechnology and Laboratory Science in Medicine (Master)

National Yang Ming University, Taipei, Taiwan.

Department of Biological Sciences and Technology (Bachelor)

National University of Tainan, Tainan, Taiwan.

Academic Awards：

1. 2021 Faculty of Medicine Faculty Academic Excellence Award, National Yang Ming Chiao Tung University
2. The society of Chinese natural medicine: Outstanding Natural Medicine Academic Paper Award-2020 Young Scholars Award
3. 107-109 Lecturer-level Research and Advancement Award (沈力揚教授醫學教育獎學紀念基金會)
4. 2019 Faculty of Medicine Faculty Academic Excellence Award, National Yang Ming University
5. 2018 MOST Young Scholar Fellowship

Research Expertise:

1. Cancer Biology
2. Protein Degradation Pathways
3. Signal Transduction

Research Topics:

The functions and mechanisms of herbal medicine suppressed cancer progression and metastasis

1. Fungal immunomodulation proteins (FIPs) from Ganoderma
2. Sulfated polysaccharides from medical fungi and marine organisms

Courses:

1. Special Topic on Protein Degradation: The mechanisms of Chinese herbal medicine on diseases therapy / The applications of Chinese herbal medicine on cancer therapy
2. Seminar
3. Analysis and Reading Guidance of Herbal Medicine-based Papers
4. Special Topic on Evidence-based Chinese Herbal Medicine

Academic Works-

Scholar Google

• Papers-Ganoderma Proteins

1. Ai-Jung Tseng, Tsung-Hsi Tu, Wei-Jyun Hua, Hsin Yeh, Ching-Jung Chen, Zhi-Hu Lin, Wei-Hung Hsu, Ying-Lan Chen, Chuan-Chih Hsu, Tung-Yi Lin*. GMI, Ganoderma microsporum protein, suppresses cell mobility and increases temozolomide sensitivity through induction of Slug degradation in glioblastoma multiforme cells. International Journal of Biological Macromolecules. 2022. 219: 940-948. (SCI, IF: 8.025, Rank: 6.7%) Corresponding author
2. Ha Phan Thanh Ho, Tung-Yi Lin, Jo-Ning Hung, Ya-Hui Chiu, Ming-Han Tsai*. Ganoderma microsporum immunomodulatory protein acts as a multifunctional broad-spectrum antiviral against SARS-CoV-2 by interfering virus binding to the host cells and spike-mediated cell fusion. Biomedicine & Pharmacotherapy. 2022. 155:113766. (SCI, IF: 7.419, Rank: 9.3%) Third author
3. Hsin Yeh, Di Ngoc Kha Vo, Zhi-Hu Lin, Ha Phan Thanh Ho, Wei-Jyun Hua, Wei-Lun Qiu, Ming-Han Tsai, Tung-Yi Lin*. GMI, a protein from Ganoderma microsporum, induces ACE2 degradation to alleviate infection of SARS-CoV-2 Spike-pseudotyped virus. 2022. 103:154215. (SCI, IF: 6.656, Rank: 3.3%) Corresponding author
4. Tung-Yi Lin*, Wei-Jyun Hua, Hsin Yeh, Ai-Jung Tseng. Functional proteomic analysis reveals that fungal immunomodulatory protein reduced expressions of heat shock proteins correlates to apoptosis in lung cancer cells. 2021. 80:153384. (SCI, IF: 6.656, Rank: 3.3%) First & Corresponding author
5. Tung-Yi Lin, Hsien-Yeh Hsu, Wei-Hsuan Sun, Tsung-Han Wu and Shu-Ming Tsao. Induction of Cbl-dependent epidermal growth factor receptor degradation in Ling Zhi-8 suppressed lung cancer. International Journal of Cancer. 2017. 140(11):2596-2607. (SCI, IF: 7.316, Rank: 21.2%) First author
6. Tung-Yi Lin and Hsien-Yeh Hsu. Ling Zhi-8 reduces lung cancer mobility and metastasis through disruption of focal adhesion and induction of MDM2-mediated Slug degradation. Cancer Letters. 2016. 375(2):340-348. (SCI, IF: 9.756, Rank: 14.3%) First author
7. Hsien-Yeh Hsu*, Tung-Yi Lin*, Ruey-Shyang Hseu, and Ching-Tsan Huang. Ling Zhi immuno-modulation protein, LZ-8 induction of interleukin-1 expression in human monocytes via pertussis toxin-sensitive G protein-mediated protein kinase pathway. Adaptive Medicine. 2014. 6(2): 63-72. Co-first author
8. Hsien-Yeh Hsu, Yen-Chou Kuan, Tung-Yi Lin, Shu-Ming Tsao, Jason Hsu, Li-Juan Ma, and Fuu Sheu. Reishi protein LZ-8 induces FOXP3⁺ Treg expansion via a CD45-dependent signaling pathway and alleviates acute intestinal inflammation in mice. Evid Based Complement Alternat Med. 2013. 2013:513542. (SCI, IF: 2.650, Rank: 53.3%)
9. Chien-Ting Wu*, Tung-Yi Lin*, Hsien-Yeh Hsu, Fuu Sheu, Chau-Mei Ho and Edmund I.-T.Chen. Ling Zhi-8 mediates p53-dependent growth arrest of lung cancer cells proliferation via the ribosomal protein S7-MDM2-p53 pathway. Carcinogenesis. 2011. 32(12):1890-1896. (SCI, IF: 4.741, Rank: 42.0%) Co-first author

• Papers-Ganoderma Polysaccharides

1. Wei-Lun Qiu, Wei-Hung Hsu, Shu-Ming Tsao, Ai-Jung Tseng, Zhi-Hu Lin, Wei-Jyun Hua, Hsin Yeh, Tzu-En Lin, Chien-Chang Chen, Li-Sheng Chen, Tung-Yi Lin*. WSG, a glucose-rich polysaccharide from Ganoderma lucidum, combined with cisplatin potentiates inhibition of lung cancer in vitro and in vivo. 2021. 13:4353. (SCI, IF: 4.967, Rank: 17.8%) Corresponding author
2. Wei-Hung Hsu, Wei-Jyun Hua, Wei-Lun Qiu, Ai-Jung Tseng, Hsin-Chung Cheng, Tung-Yi Lin*. WSG, a glucose-enriched polysaccharide from Ganoderma lucidum, suppresses tongue cancer cells via inhibition of EGFR-mediated signaling and potentiates cisplatin-induced apoptosis. International Journal of Biological Macromolecules. 2021. 193:1201-1208. (SCI, IF: 8.025, Rank: 6.7%) Corresponding author
3. Wei-Hung Hsu, Wei-Lun Qiu, Shu-Ming Tsao, Ai-Jung Tseng, Mei-Kuang Lu, Wei-Jyun Hua, Hsin-Chung Cheng, Hsien-Yeh Hsu, Tung-Yi Lin*. Effects of WSG, a polysaccharide from Ganoderma lucidum, on suppressing cell growth and mobility of lung cancer. International Journal of Biological Macromolecules. 2020. 165:1604-1613. (SCI, IF: 8.025, Rank: 6.7%) Corresponding author
4. Shih-Fen Liao, Chi-Hui Liang, Ming-Yi Ho, Tsui-Ling Hsu, Tsung-I Tsai, Yves S.-Y. Hsieh, Chih-Ming Tsai, Shiou-Ting Li, Yang-Yu Cheng, Shu-Ming Tsao, Tung-Yi Lin, Zong-Yan Lin, Wen-Bin Yang, Chien-Tai Ren, Kuo-I Lin, Kay-Hooi Khoo, Chun-Hung Lin, Hsien-Yeh Hsu, Chung-Yi Wu, and Chi-Huey Wong. Immunization of fucose-containing polysaccharides from Reishi mushroom induces antibodies to tumor-associated Globo H-series epitopes. Proc Natl Acad Sci U S A. 2013. 110(34):13809-14. (SCI, IF: 12.779, Rank: 12.3%)

• Papers-Antrodia cinnamomea Polysaccharides

1. Tung-Yi Lin*, Mei-Kuang Lu, Ai-Jung Tseng, Chi-Hsein Chao. Effects of sterol-type elicitors on biochemical characterization of polysaccharides from Antrodia cinnamomea. International Journal of Biological Macromolecules. 2020. 162:1476-1483. (SCI, IF: 8.025, Rank: 6.7%) First & Corresponding author
2. Tung-Yi Lin*, Chia-Chuan Chang, Ai-Jung Tseng, Chi-Hsein Chao, Mei-Kuang Lu. Sodium thiosulfate enhances production of polysaccharides and anticancer activities of sulfated polysaccharides in Antrodia cinnamomea. Carbohydrate Polymers. 2019. 216:204-212. (SCI, IF: 10.723, Rank: 3.3%) First & Corresponding author
3. Tung-Yi Lin*, Ai-Jung Tseng, Wei-Lun Qiu, Chi-Hsein Chao, Mei-Kuang Lu. A sulfated glucan from Antrodia cinnamomea reduces Slug expression through regulation of TGFβ/AKT/GSK3β axis in lung cancer. Carbohydrate Polymers. 2019. 210:175-184. (SCI, IF: 10.723, Rank: 3.3%) First & Corresponding author
4. Mei-Kuang Lu*, Tung-Yi Lin*, and Chia-Chuan Chang. Chemical identification of a sulfated glucan from Antrodia cinnamomea and its anti-cancer functions via inhibition of EGFR and mTOR activity. Carbohydrate Polymers. 2018. 202:536-544. (SCI, IF: 10.723, Rank: 3.3%) Co-first author
5. Tung-Yi Lin*, Ai-Jung Tseng, Chi-Hsein Chao, Mei-Kuang Lu. Microelements induce changes in characterization of sulfated polysaccharides from Antrodia cinnamomea. International Journal of Biological Macromolecules. 2018. 120:952-958. (SCI, IF: 8.025, Rank: 6.7%) First & Corresponding author
6. Mei-Kuang Lu*, Tung-Yi Lin*, Chun-Hao Hu, Chi-Hsein Chao, Chia-Chuan Chang, and Hsien-Yeh Hsu. Characterization of a sulfated galactoglucan from Antrodia cinnamomea and its anticancer mechanism via TGFβ/FAK/Slug axis suppression. Carbohydrate Polymers. 2017. 167: 229–239. (SCI, IF: 10.723, Rank: 3.3%) Co-first author
7. Mei-Kuang Lu*, Tung-Yi Lin*, Chi-Hsein Chao, Chun-Hao Hu and Hsien-Yeh Hsu. Molecular mechanism of Antrodia cinnamomea Sulfated polysaccharide on the suppression of lung cancer cell growth and migration via induction of transforming growth factor β receptor degradation. International Journal of Biological Macromolecules. 2017. 95:1144–1152. (SCI, IF: 8.025, Rank: 6.7%) Co-first author

• Papers-Fucoidan

1. Wei-Lun Qiu, Ai-Jung Tseng, Hsien-Yeh Hsu, Wei-Hung Hsu, Zhi-Hu Lin, Wei-Jyun Hua, Tung-Yi Lin*. Fucoidan increased the sensitivity to gefitinib in lung cancer cells correlates with reduction of TGFβ-mediated Slug expression. International Journal of Biological Macromolecules. 2020. 153:796-805. (SCI, IF: 8.025, Rank: 6.7%) Corresponding author
2. Hsien-Yeh Hsu*, Tung-Yi Lin*, Chun-Hao Hu, David Ta Fu Shu, Mei-Kuang Lu. Fucoidan Upregulates TLR4/CHOP-Mediated Caspase-3 and PARP Activation to Enhance Cisplatin-Induced Cytotoxicity in Human Lung Cancer Cells. Cancer Letters. 2018. 432:112-120. (SCI, IF: 9.756, Rank: 14.3%) Co-first author
3. Hsien-Yeh Hsu*, Tung-Yi Lin*, Shu-Ming Tsao, Mei-Kuang Lu, Pei-Ju Leng, and Yu-Chung Wu. Fucoidan induces Toll-like receptor 4-regulated reactive oxygen species and promotes endoplasmic reticulum stress-mediated apoptosis in lung cancer. Scientific Reports. 2017. 7:44990. (SCI, IF: 4.996, Rank: 26.0%) Co-first author
4. Hsien-Yeh Hsu*, Tung-Yi Lin*, Yu-Chung Wu, Shu-Ming Tsao, Pai-An Hwang, Yu-Wei Shih and Jason Hsu. Fucoidan inhibition of lung cancer in vivo and in vitro: role of the Smurf2-dependent ubiquitin proteasome pathway in TGFβ receptor degradation. Oncotarget. 2014. 5(17):7870-85. Co-first author
5. Hsien-Yeh Hsu*, Tung-Yi Lin*, Pai-An Hwang, Ling-Ming Tseng, Ren-Hao Chen, Shu-Ming Tsao and Jason Hsu. Fucoidan induces changes in the epithelial-mesenchymal transition and decreases metastasis by enhancing ubiquitin-dependent TGFβ receptor degradation in breast cancer. Carcinogenesis. 2013. 34(4):874-84. (SCI, IF: 4.741, Rank: 42.0%) Co-first author

• Papers-Polysaccharides from various natural products

1. Tung-Yi Lin, Mei-Kuang Lu, Chia-Chuan Chang*. Structural identification of a fucose-containing 1,3-β-mannoglucan from Poria cocos and its anti-lung cancer CL1-5 cells migration via inhibition of TGFβR-mediated signaling. International Journal of Biological Macromolecules. 2020. 157:311-318. (SCI, IF: 8.025, Rank: 6.7%) First author

• Papers-Herbal Formula

1. Yueh-Hsin Ping, Hsin Yeh, Li-Wei Chu, Zhi-Hu Lin, Yin-Chieh Hsu, Lie-Chwen Lin, Chung-Hua Hsu, Shu-Ling Fu, Tung-Yi Lin*. The Traditional Chinese Medicine Formula Jing Guan Fang for preventing SARS-CoV-2 infection: from clinical observation to basic research. Frontiers in Pharmacology. 2022. 13:744439. (SCI, IF: 5.988, Rank: 17.9%) Corresponding author
2. Kai-Wei Chang, Tung-Yi Lin, Shu-Ling Fu, Yueh-Hsin Ping, Fen-Pey Chen, Yen-Ying Kun*. A Houttuynia Cordata-based Chinese herbal formula improved symptoms of allergic rhinitis during COVID-19 pandemic. Journal of the Chinese Medical Association. 2022. 85(6):717-722. (SCI, IF: 3.396, Rank: 41.3%) Second author
3. Zhi-Hu Lin, Jinsong Hu, Huagang Shi, Chia-Ching Liaw, Wei-Lun Qiu, Wei-Hung Hsu, Tung-Yi Lin*. Water extract of medicinal ink (WEMI) attenuates lipopolysaccharide-induced NO production of Raw264. 7 cells via downregulation JAK2/STAT3-mediated iNOS expression. Journal of Ethnopharmacology. 2022. 282:114636. (SCI, IF: 5.195, Rank: 13.3%) Corresponding author

• Papers-Biomedical Engineering

1. Wei-Shan Hsu, Anant Preet, Tung-Yi Lin and Tzu-En Lin*. Miniaturized Salinity Gradient Energy Harvesting Devices. Molecules. 2021, 26(18): 5469. (SCI, IF: 4.927, Rank: 36.3%) Third author
2. Tung-Yi Lin, Kuan-Yi Lee, Tsui-Ling Chang, Chia-Chin Chang, Yen-Zhu Lin. The electrochemical method for detecting 26S proteasome. Sensors and Actuators B. 2011. 160:412-417. (SCI, IF: 9.221, Rank: 3.1%) First author
3. Tung-Yi Lin, Kuan-Yi Lee, Tsui-Ling Chang and Chia-Chin Chang. The electrolyte effect on adsorption of 1,6-Hexanedithiol at gold electrode. National University of Tainan, Journal of Ecology and Environmental Sciences. 2010. 3(1): 32-45. First author

• Papers-Sport Science

1. Yun-Tsung Chen, Yao-Yi Hsieh, Jen-Yu Ho, Chien Chang Ho, Tung-Yi Lin* and Jung-Charng Lin. Running interval training combined with blood flow restriction increases maximal running performance and muscular fitness in male runners. Scientific reports. 12:9922. (SCI, IF: 4.996, Rank: 26.0%) Corresponding author
2. Yun-Tsung Chen, Yao-Yi Hsieh, Jen-Yu Ho, Tung-Yi Lin* and Jung-Charng Lin. Two weeks of detraining reduces cardiopulmonary function and muscular fitness in endurance athletes. European journal of sport science. 2022. 22(3):399-406. (SCI, IF: 3.980, Rank: 27.6%) Corresponding author
3. Yun-Tsung Chen, Yao-Yi Hsieh, Jen-Yu Ho, Tung-Yi Lin and Jung-Charng Lin. Running training combined with blood flow restriction increases cardiopulmonary function and muscle strength in endurance athletes. Journal of strength and conditioning research. 2022. 36(5):1228-1237. (SCI, IF: 4.415, Rank: 20.7%)

Conference:

1. Wei-Jyun Hua, Ai-Jung Tseng, Tsung-His Tu, Tung-Yi Lin*. Ganoderma microsporum protein (GMI) exerts anti-motility activity and potentiates temozolomide efficacy via induction of Slug degradation in glioblastoma multiforme cells. The 37^th Symposium on Natural Products (NO: NPB02). Kaohsiung Medical University, Taiwan, 2022.
2. Hsin Yeh, Di Ngoc Kha Vo, Zhi-Hu Lin, Ha Phan Thanh Ho, Wei-Jyun Hua, Wei-Lun Qiu, Ming-Han Tsai, Tung-Yi Lin*. GMI, an immunomodulatory protein from Ganoderma microsporum, prevents SARS-CoV-2 Spike-pseudotyped virus infection through inducing ACE2 degradation. The 37th Symposium of Natural Products (NO: NPC02). Kaohsiung Medical University, Taiwan, 2022.
3. Wei-Lun Qiu, Wei-Hung Hsu, Tung-Yi Lin*. A water-soluble glucose-rich polysaccharide from Ganoderma lucidum sensitizes lung cancer cells to cisplatin through inducing apoptosis. The 37^th Symposium of Natural Products (NO: NPA19). Kaohsiung Medical University, Taiwan, 2022.
4. Hung-Chih Lo, Hsien-Yeh Hsu, Mei-Kuang Lu, Tung-Yi Lin*. Using Tangential Flow Filtration System to optimize the anti-cancer activity of polysaccharide from Laminaria japonica. The 37^th Symposium of Natural Products (NO: NPA18). Kaohsiung Medical University, Taiwan, 2022.
5. Zhi-Hu Lin, Mei-Kuang Lu, Ai-Jung Tseng, Chi-Hsein Chao, and Tung-Yi Lin*. Sulfated polysaccharides from Antrodia cinnamomea inhibits lung cancer cells via induction of apoptosis and activation of macrophages. 14^th Meeting of the Asia Pacific Federation of Pharmacologists. (NO: EO83). Taipei International Convention Center, Taiwan, 2021.
6. Wei-Jyun Hua, Wei-Hung Hsu and Tung-Yi Lin*. Effects of polysaccharide from Ganoderma lucidum on suppressing oral squamous cell carcinoma growth by regulating EGFR/AKT signaling and induction of apoptosis. The 36^th Symposium of Natural Products (NO: NPC04). National Sun Yat-sen University, Taiwan, 2021.
7. Wei-Lun Qiu, Chuang-Ching Chen, and Tung-Yi Lin*. Anti-colon cancer effect of Ganoderma microsporum immunomodulatory protein combined with 5-Flurouracie. The 36^th Symposium of Natural Products (NO: PHA07). National Sun Yat-sen University, Taiwan, 2021.
8. Zhi-hu Lin, Chia-Ching Liaw, Wei-Lun Qiu, Wei-Hung Hsu and Tung-Yi Lin*. Water extract of medicinal ink (WEMI) presents anti-inflammatory effects on LPS-induced Raw264.7 cells via regulation of JAK2/STAT3 signaling pathway. The 36^th Symposium of Natural Products (NO: TCM25). National Sun Yat-sen University, Taiwan, 2021.
9. Li-Chen Huang, Wei-Jyun Hua and Tung-Yi Lin*. GMI induces proteasome-dependent PD-L1 degradation via regulating GSK3β pathway in lung cancer cells. The 36^th Symposium of Natural Products (NO: PHA18). National Sun Yat-sen University, Taiwan, 2021.
10. Zhi-hu Lin, Wei-Hung Hsu, and Tung-Yi Lin*. The anti-inflammatory effects of Jinchuang Ointment on LPS-induced Raw 264.7 cells. The 35^th Symposium of Natural Products (NO: C-05). Taipei Medical University, Taiwan, 2020.
11. Tung-Yi Lin* and Mei-Kuang Lu. Squalene induces changes in biochemical characterization of polysaccharides from Antrodia cinnamomea. The 35^th Symposium of Natural Products (NO: C-08). Taipei Medical University, Taiwan, 2020.
12. Hsin Yeh, Wei-Jyun Hua, and Tung-Yi Lin*. Fungal immunomodulatory proteins downregulate expression of heat shock proteins in lung carcinoma LLC1 cells. The 35^th Symposium of Natural Products (NO: B-22). Taipei Medical University, Taiwan, 2020.
13. Wei-Hung Hsu, Wei-Lun Qiu, Chung-Hua Hsu, Tung-Yi Lin*. Characterization of a glucose-rich polysaccharide from Ganoderma lucidum on suppression of lung cancer and enhancement of cisplatin-induced cytotoxicity.12^th Taipei Traditional Chinese Medicine International Forum 2020 (NO: TICMIF-2020-PO42). NTUH International Convention Center, Taiwan, 2020.
14. Wei-Lun Qiu and Tung-Yi Lin*. Fucoidan suppressed TGFβ-mediated molecules correlated with the sensitive enhancement of gefitinib in lung cancer cells. The 35^th Symposium of Natural Products (NO: B-10). Taipei Medical University, Taiwan, 2020.
15. Tung-Yi Lin* and Mei-Kuang Lu. Sulfated polysaccharides from Antrodia cinnamomea cultured with Sodium thiosulfate enhanced synergistically clinical drug-induced cytotoxic effects in TKI-resistant lung cancer H1975 cells. The 34^th Symposium of Natural Products (NO: NPP-36). Chang Gung University of Science and Technology, Taiwan, 2019.
16. Tung-Yi Lin, Mei-Kuang Lu and Chia-Chuan Chang. Mannoglucan from Poria cocos and its anti-cancer related activities. The 34^th Symposium of Natural Products (NO: NPC-22). Chang Gung University of Science and Technology, Taiwan, 2019.
17. Ai-Jung Tseng, Tsung-His Tu and Tung-Yi Lin*. GMI, a protein from Ganoderma microsporum, suppresses cell viability and migration via inhibition of EGFR-mediated signaling in glioblastoma multiforme GBM8401 cells. 2019 Southeast Asia Symposium of Traditional Medicine (NO: BR-02), National Yang-Ming University, Taiwan, 2019.
18. Wei-Jyun Hua and Tung-Yi Lin*. Fungal immunoregulatory protein, GMI, suppresses cell viability and mobility in lung squamous carcinoma cells. 2019 Southeast Asia Symposium of Traditional Medicine (NO: BR-15), National Yang-Ming University, Taiwan, 2019. (Excellent Poster Award)
19. Tung-Yi Lin*, Mei-Kuang Lu. A sulfated glucan from Antrodia cinnamomea suppresses lung cancer: blockage of TGFβ-mediated signalings controls Slug stability. The 33th Symposium of Natural Products (NO: NP-8). Kaohsiung Medical University, Taiwan,
20. 林東毅、李冠誼、張家欽、張翠玲。26S proteasome偵測系統中電解液對於黃金電極吸附HDT影響之研究。中國材料年會 (論文編號: 06-0612)，國立東華大學，2009。

Patents and know-how:

1. Hsien-Yeh Hsu and Tung-Yi Lin. Method for treating a refractory or relapsed lung cancer. US 9,827,288 B2. 2017. (徵求技術轉移)
2. Tung-Yi Lin and Mei-Kuang Lu. Composition and method for preventing or treating cancer. Provisional application: 63/283,420. 2021. (徵求技術轉移或讓與)
3. Chung-Hua Hsu, Shu-ling Fu, Tung-Yi Lin, Yueh-Hsin Ping. Herbal composition, preparation method thereof and method for preventing or treating respiratory disorder by administering the same. Provisional application: 63/280,635. 2021. (徵求技術轉移或讓與)
4. Tung-Yi Lin and Ming-Han Tsai. An Immunomodulatory protein for prevention and/or treatment of coronavirus infection. Provisional application: 63/266,712. 2022. (徵求技術轉移)
5. Tung-Yi Lin. An Immunomodulatory protein for alleviation and/or treatment of coronavirus disease. Provisional application: 63/266,716. 2022. (徵求技術轉移)
6. Tung-Yi Lin. Method for treating PD-L1 expressing cancer. Provisional application: 63/303,523. 2022. (徵求技術轉移或讓與)

–Research Project

-(Please Provide A 5-Year Plan)

Project:

Ministry of Science and Technology:

1. MOST Grant for the Einstein Program: Combination of cancer targeted and immune therapy: Investigation of the dual effects and mechanisms of Ganoderma proteins on anti-cancer and activation of immunity in tumor-microenvironment of lung cancer.09.01-2023.08.31. Principal Investigator
2. Effects of Fungal Immunomodulatory Protein in Spinal Cord Injury: Focused on Inflammation and Neuroregeneration. 08.01-2021.07.31。Sub-Principal Investigator

Ministry of Health and Welfare:

1. Investigation of the efficacy and mechanisms of Ganoderma lucidum polysaccharides combined with chemotherapeutic drug on inhibition of lung cancer cells. 2022.01.01-2022.12.31. Co-Principal Investigator
2. Investigation of the efficacy and mechanisms of Chinese herbal-based mouthwash: Shu-Yi-Xiao on protection of oral cells and anti-inflammation. 2021.01.01-2021.12.31. Co-Principal Investigator
3.  Investigation of the efficacy and mechanisms of extraction from Ganoderma lucidum on promoting wound healing for patients with Hansen’s disease. 2020.01.01-2020.12.31. Co-Principal Investigator

 Veterans General Hospitals and University System of Taiwan Joint Research Program:

1. Study on the molecular mechanisms of targeting HGFR and Slug expressions in Ganoderma immunomodulatory protein inhibited Glioblastoma Multiforme cells. 2021.01.01-2021.12.31. Principal Investigator

Others:

1.  New Taipei city Chinese medical doctors’ association: Investigation of anticancer functions and mechanisms of glucan from Ganoderma lucidum. 2020.01.01-2020.12.31. Co-Principal Investigator

※目前研究主軸：

1.靈芝類免疫調節小分子蛋白【GMI、LZ-8】
2.多醣體【靈芝多醣體、牛樟芝多醣體、褐藻醣膠】
3.中草藥複方 

1.靈芝類免疫調節小分子蛋白【GMI、LZ-8】
靈芝類免疫調節蛋白(包含LZ-8或GMI)是從靈芝類菌絲體所萃取之小分子蛋白質，且被認為具有高度潛力開發成新穎蛋白藥物的特殊小分子。我們團隊在先前的相關研究已經證實此類蛋白具有免疫調節的功效(Evid Based Complement Alternat Med. 2013. 2013:513542.; Adaptive Medicine. 2014. 6(2): 63-72.)；除此之外，我們進一步剖析此類蛋白對於癌細胞的影響與相關機制之研究。我們發現LZ-8誘發RP-MDM2-P53訊號路徑進而可以抑制肺癌細胞的增生，並於小鼠實驗中證實LZ-8可以有效地降低腫瘤大小(Carcinogenesis. 2011. 32(12):1890-6.)；我們也發現LZ-8可以抑制癌細胞內黏著斑激酶(FAK)活性並誘導上皮-間質細胞轉換 (epithelial to mesenchymal transition, EMT)重要的轉錄因子SLUG進行泛素化依賴性降解作用(Ubiquitination-dependent degradation)，此過程可有效地抑制癌細胞移動並且抑制癌症小鼠模式中的癌細胞轉移之能力(Cancer Letters. 2016. 375(2):340-348.)。除此之外，肺癌是目前國內以及世界上癌症得病率且致死率皆很高的一個疾病，其中肺癌細胞中一個特殊蛋白質：突變型表皮生長因子受器(EGFR)，是目前被臨床作為抗肺癌的重要標的物；而我們的研究也於世界上首次證實靈芝類蛋白藥物可以誘導泛素化依賴性之EGFR降解作用，從而造成癌細胞生長停滯或者凋亡現象(International Journal of Cancer. 2017. 140(11):2596-2607.)。近來，我們更剖析癌症小鼠給予靈芝類蛋白藥物後所造成之腫瘤內蛋白質體之變化，並透過體外試驗證實靈芝蛋白抑制癌細胞之新穎調控路徑(Phytomedicine. 2021. 80:153384.)。

目前，我們仍繼續專注探討此靈芝類蛋白之抗癌與免疫調節功效與作用機轉，並著重於肺癌(肺腺癌、肺鱗狀細胞癌)、腦癌、乳癌、大腸癌與抗發炎等相關研究。目前我們主要對於靈芝蛋白的研究主要進行六大方向探討：

第一，探討靈芝蛋白影響各式種類癌細胞之全基因表現，並分析特定基因族群對於癌細胞之影響。

第二，探討靈芝蛋白調節癌細胞中致癌及免疫抑制相關蛋白之穩定性的重要機制。

第三，探討靈芝蛋白合併臨床用藥：標靶藥物、化療藥物與癌症免疫藥物之藥物加成作用功效與機制。

第四，探討靈芝蛋白於癌細胞與免疫細胞之重要標的蛋白，並開發特殊標的蛋白之抑制劑或拮抗劑。

第五，研究靈芝蛋白誘導細胞壓力(cellular stress)與細胞自噬作用(autophagy)進而抑制癌細胞增生與轉移相關機制研究。

第六，探討靈芝蛋白於抗病毒感染作用與機轉。

我們研究團隊將持續研究靈芝蛋白，希冀未來其可具有潛能被開發為抗人類癌細胞增長與其轉移的重要新分子藥物，以及新穎抗病毒用藥。

 2.多醣體【靈芝多醣體、牛樟芝多醣體、褐藻醣膠】

◎靈芝多醣體
靈芝，從古至今一直皆是大家嚮往之神草，亦被神農本草經列為上品藥物。靈芝中的多醣體成份已被證實具有抗癌、免疫調節(Proc Natl Acad Sci U S A. 2013. 110(34):13809-14.)、改變腸道菌相等功能；現今，市面上靈芝多醣體也具有許許多多相關產品且被作為保健食品。然而靈芝多醣體是一個非常複雜之大分子多醣體，且其抗癌的相關機制並不是很清楚。我們團隊首度證實特定靈芝多醣體WSG之化學特性，並證實其有效地抑制肺癌與口腔癌之能力(International Journal of Biological Macromolecules. 2020. 165:1604-1613.; 2021. 193: 1201-1208.)。此外，我們更鑑定了靈芝多醣體WSG合併化療用藥cisplatin可以有效抑制肺癌增長與轉移(Polymers. 2021. 13 (24), 4353.)。為更加了解靈芝多醣的相關抗癌機轉，我們目前亦著重在來自靈芝菌絲體中的多醣體研究包括：

第一，系統性地建立靈芝菌絲體培養，並配合一系列特殊多醣體萃取流程技術後，我們將萃取之靈芝多醣體更進一步純化分離，找出具有生物活性之重要成分。

第二，分析活性靈芝多醣體成份與結構。

第三，探討靈芝多醣體有效成分之抗癌機轉，並著重於癌細胞中致癌蛋白之降解過程。

第四，探討靈芝多醣體合併臨床用藥：標靶藥物、化療藥物與癌症免疫藥物之藥物加成作用功效與機制。

第五，探討靈芝多醣體影響受傷組織修復之功能與機制探討。

    我們研究團隊從靈芝菌絲體培養做起，經由一系列品管過程，確保多醣體之來源與品質，並持續找尋有效之靈芝多醣體，希冀未來可以開發出具有特定結構之活性多醣體並探討其抗癌、疾病相關機制，提供靈芝多醣體之活性成分之重要科學實證。

◎牛樟芝多醣體
牛樟芝為臺灣特有種，具有抗發炎、保肝、抗癌等作用，被譽為臺灣國寶。牛樟芝中的有效多醣體成份是我們團隊特別著重探討之重要議題。我們獨步全球首先證實牛樟芝中硫化多醣體的抗癌分子機轉(International Journal of Biological Macromolecules. 2017. 95:1144–1152.)。我們更進一步分離出各式各樣的特定結構之硫酸化葡聚醣(Sulfated glucan)，並分析其抗癌效果也許可經由許多不同細胞反應，如抑制EGFR活性或者誘導泛素化依賴性之轉化生長因子受器(TGFβR)、SLUG降解作用等(Carbohydrate Polymers. 2017. 167: 229–239.; 2018. 202:536-544.; 2019. 210:175-184.)。除此之外，我們更領先探討植物用肥料與微量元素甚至臨床化學用藥對於牛樟芝硫化多醣體之成份與活性功能之分析(International Journal of Biological Macromolecules. 2018. 120:952-958.; Carbohydrate Polymers. 2019. 216:204-212.)。我們團隊目前仍持續著重在純化各式具有抗癌與免疫調節的活性多醣體，研究包括：

第一，探討各式各樣化學試劑與植物用肥料等對於牛樟芝生長與菌絲體內多醣體建構及組成之改變。

第二，純化與分析有效活性牛樟芝多醣體成份與結構。

第三，探討牛樟芝多醣體有效成分之抗癌機轉，並著重於癌細胞中各式細胞反應與致癌基因的改變。

第四，探討牛樟芝多醣體有效成分之抗發炎機轉，並著重於免疫細胞中各式細胞反應與癌症免疫治療之策略。

第五、探討牛樟芝具活性多醣體合併臨床用藥：標靶藥物、化療藥物與癌症免疫藥物之藥物加成作用功效與機制。

    我們研究團隊將持續找尋有效提升牛樟芝硫化多醣體之物質，並純化具有高度活性之特定結構多醣體，希冀未來可以應用於牛樟芝多醣體產品之商業開發，並研究相關抗癌與抗發炎機制，提供牛樟芝多醣體之活性成分之重要科學實證。

◎褐藻醣膠
來自海藻中的岩藻糖聚體，稱之為褐藻醣膠(fucoidan)，係近年來全世界大力開發的海藻多醣體萃取物，其在免疫調節與抗癌功效已有許多研究證實。我們先前研究發現褐藻醣膠抑制癌細胞的生長、轉移及侵襲能力之作用機制係誘導泛素化依賴性之TGFβR降解作用等(Carcinogenesis. 2013. 34(4):874-84.; Oncotarget. 2014. 5(17):7870-85.)。我們也發現褐藻醣膠造成癌細胞的反應現象可能透過特殊膜蛋白(TLR4)之影響(Scientific Reports. 2017. 7:44990.)。另外，我們近期發現，褐藻醣膠可以做為化療藥物之輔助劑，不只與化療藥物合併使用可以有加成作用，更可降低化療和標靶藥物使用劑量(Cancer Letters. 2018. 432:112-120.; International Journal of Biological Macromolecules. 2020. 153:796-805.)。我們團隊目前仍持續著重在探討褐藻醣膠之抗癌作用，研究包括：

第一，探討褐藻醣膠於各式癌細胞中之抗癌作用與機制探討。

第二，探討褐藻醣膠影響各式種類癌細胞之全基因表現，並分析特定基因族群對於癌細胞之影響。

第三，探討褐藻醣膠合併臨床用藥：標靶藥物、化療藥物與癌症免疫藥物之藥物加成作用功效與機制。

第四，探討褐藻醣膠誘導活化免疫細胞，強化癌症免疫治療之功效

第五，探討褐藻醣膠強化受傷組織修復之功能與機制探討。

    我們希冀藉由對於褐藻醣膠之相關研究可以提供之重要科學證據應用於褐藻醣膠產品之商業或臨床應用開發。

※學術單位合作：
「多醣體開發研究團隊」
我們與衛生福利部國家中醫藥研究所 盧美光研究員以及國立臺灣大學藥學系 張嘉銓教授成立「多醣體開發研究團隊」，從事植物和真菌類特定多醣體之生產、萃取純化、結構鑑定、抗癌與抗發炎的活性分析與分子機轉探討。
「靈芝蛋白應用腦癌與神經修復研究團隊」
我們與台北榮民總醫院杜宗熹醫師團隊合作，探討靈芝類蛋白於抑制腦癌與神經修復之功能與分子機轉探討。

「靈芝蛋白抗病毒研究團隊」
我們與陽明交通大學微免所蔡明翰教授團隊合作，探討靈芝蛋白於預防各式病毒感染之功能與分子機轉探討。

「中藥複方應用於特殊疾病之研究團隊」
我們與衛生福利部樂生療養院 徐蔚泓醫師共同合作，從事探討各式中藥複方對於傷口與口腔疾病之功效與基礎研究探討。

※TLlab的成員：
研究助理：曾愛容
博士班學生：
邱偉綸、林志虎、花偉鈞、葉心
碩士班學生：
陳長青、黃麗真、羅弘智

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Email: biotungyi@gmail.com or tylin99@nycu.edu.tw

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